氣相色譜-三重四極桿串聯(lián)質(zhì)譜法測(cè)定生姜中咪鮮胺及其代謝物總殘留量的研究

打開文本圖片集

Research on Determination of Total Residues of Prochloraz and Its Metabolites in Ginger by Gas Chromatography-Triple Quadrupole Tandem Mass Spectrometry

LI Hui （Ningde Product Quality Inspection Institute，Ningde 35210o， China）

Abstract： Objective： To establish a method for determining the total residues of prochloraz and its metabolites inginger using gas chromatography-triple quadrupole tandem mass spectrometry. Method： The target analytes in ginger were extracted with acidified acetonitrile. Under the condition of 220°C air bath， the target analytes were reacted with pyridine hydrochloride to promote the complete conversion of prochloraz and its metabolites into the secondarymetabolite2，4，6-trichlorophenol.After purifcation withconcentrated sulfuricacid，thedetermination was performed by gas chromatography-mass spectrometry in multiple reaction monitoring mode，and the measured value was converted into theresidualamountof prochloraz.Result： Under theoptimizedconditions，2，4，6-trichlorophenol showed a good linear relationship in the range of 0.1～2.0μg?mL-1. ，with a correlation coefficient of 0.9996 At the three spiked concentration levels of O.05， 0.20，and 0.50mg.kg-1 ， the recoveries of prochloraz were 82.3% 0 91.0% and 96.1% respectively，with relative standard deviations of 6.1% 2.6% and 5.0% respectively. The limit of quantitation of prochloraz in ginger was 0.007 6mg?kg-1 . Conclusion： This method is rapid， simple，and easy to operate，and can meet the requirements for the detection of prochloraz and its metabolite residues in large quantities of ginger.

Keywords： gas chromatography-triple quadrupole tandem mass spectrometry; ginger; prochloraz

生姜是我國(guó)廣泛種植的重要蔬菜之一，在居民的日常飲食消費(fèi)中占據(jù)重要地位。（剩余5301字）