SPE-HPLC法測定食品中對(duì)羥基苯甲酸酯類防腐劑

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Determination of p -Hydroxybenzoate Preservatives in Foods by SPE-HPLC

GUO Fuchao， WANG Tianping，HUANG Hecheng， SUN Yayun， CAO Ailing （Yunnan Yunce Quality Testing Co.， Ltd.， Kunming 650501， China）

Abstract： A analytical method for the determination of seven kinds of p-hydroxybenzoate preservatives in foods by solid phase extraction combined with high performance liquid chromatography is established.The sample pretreatment process and detection method are improved by optimizing the solid phase extraction conditions and the separation parameters of high performance liquid chromatography. The results show that this method has good separation efect and high sensitivity for the seven common p-hydroxybenzoates in foods，the linear range is good within the range of 0.2mg-L-1 to 50mg?L-1 （the correlation coefficients are all greater than O.999）， the average recovery rate is from 82.4% to 95.8% ，therelative standard deviation is from 0.35% to 3.18% ，and the limit of detection is from 0.18mg.L-1 to 0.27mg.kg-1 ， which can meet the accurate detection requirements of p-hydroxybenzoate preservatives in foods.

Keywords： solid phase extraction; high performance liquid chromatography; food; p-hydroxybenzoate

對(duì)羥基苯甲酸酯類防腐劑，憑借廣譜抗菌特性、高效抑菌能力及低毒性優(yōu)勢(shì)，在食品與化妝品領(lǐng)域應(yīng)用廣泛。（剩余4795字）