梔子中藏紅花酸抗氧化及降糖能力研究

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Study on the Antioxidant and Hypoglycemic Abilities of Crocetin in Gardenia jasminoides Ellis

CHEN Shiyun，LYU Chenghao， QIN Si* （Hunan ， ， ）

Abstract： Objective： To investigate the antioxidant and hypoglycemic effects of crocetin in Gardenia jasminoides Elis.Method： The scavenging abilityof crocetin on ABTS+freeradicals and its inhibitory activities on （20 a -amylase and a -glucosidase were evaluated through in vitro antioxidant enzyme and digestive enzyme inhibition tests，and its hypoglycemic effect was investigated using the insulin-resistant HepG2 cell model.Result：The half maximal inhibitory concentrations （IC50） of saffron acid on ABTS+ free radicals， a -amylase， and αa -glucosidase were 16.70，35.23μg?mL-1 and 7.58μg?mL-1 ，respectively， showing a significant dose-dependent inhibitory effect; in cell experiments，crocetin significantly promoted glucose uptake in insulin resistant HepG2 cels. Conclusion： Crocetin derived from Gardenia jasminoides Elis hasantioxidantcapacityand hypoglycemic activity，which provides important theoretical basis for the development of functional foods for preventing metabolic diseases.

Keywords： Gardenia jasminoides Ellis; crocetin; antioxidant; hypoglycemic

近年來，全球范圍內(nèi)糖代謝異常相關(guān)疾病發(fā)病率呈逐年顯著上升趨勢，已對公眾健康構(gòu)成重大威脅[1]。（剩余4979字）