高效液相色譜法測(cè)定食品中多種添加劑的方法研究

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Study on High Performance Liquid Chromatography Method for Determination of Multiple Additives in Food

CAO Qi

（Xinjiang Production and Construction Corps Xingxin Vocational and Technical College，Tiemenguan 84107，China）

Abstract： Objective： To establish a high performance liquid chromatography method for the detection of preservatives，sweeteners and colorants in various food substrates based on the urgent need offood safety monitoring. Method： Preservatives were separated by C18 column combined with methanol-water system， sweeteners were separated by TSKgel Amide-80 column combined with acetonitrile-water system，and quantitative analysis was performed by UV detector. Result： The linear relationship between the target compounds was good in the range of 0.1mg?L-1 to 10.0mg.L-1 ，the correlation coefficient was greater than 0.999，and the limit of detection was 0.005mg?kg-1 to 0.015mg?kg-1 ，and limit of quantitation was 0.015mg.kg-1 to 0.045mg.kg-1 . Three representative matrices including carbonated beverage， preserved fruit and sausage were selected for validation.The recoveries were 84.5% to 95.6% . Conclusion： The method can detect 6 common food additives at the same time， and has good accuracy and precision，which can meet the daily monitoring needs offood safety，and can provide reliable technical support for the supervision department.

Keywords： high performance liquid chromatography; food additives; residue analysis; method application; detection conditions

食品添加劑是食品生產(chǎn)加工過程中使用的重要物質(zhì)，在改善食品品質(zhì)與延長(zhǎng)保質(zhì)期等方面發(fā)揮著關(guān)鍵作用[1-2]。（剩余2930字）