聚多巴胺功能化普魯士藍用于黃曲霉毒素 B1 適體傳感研究
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Polydopamine-Functionalized Prussian Blue for Aptamer Sensing of Aflatoxin B1
JIANG Ziping， CHEN Jiajie，BIN Xin， ZHAO Qian*， SHI Xingbo （College ofFood Science and Technology，Hunan Agricultural UniversityHunan Provincial KeyLaboratoryofFood Science and Biotechnology， Changsha ， China）
Abstract： This article improves the traditional enzyme-linked immunosorbent assay and establishes a novel immunoassay technology based on enriched polydopamine Prusian blue nanoenzyme and adapter binding for high-sensitivity detection of aflatoxin B1 （AFT B1， ）. The results showed that the immunoprobes functionalized with polydopamine and binding to adapter Prussian blue were successfully synthesized; under the optimal reaction conditions， the regression equation for the AFT B1 colorimetric signal is A652nm=0.101gc（AFTB1）+0.72 ，with a detection limit of 0.22pg?mL-1 ， verifying the good specificity of this method; In the detection of AFT ΔB1 in rice samples， the recovery rate ranged from 96.41% to 111.19% ，indicating that this method has broad application prospects in the detection of food AFT B1 ：
Keywords： aflatoxin B1; Prussian blue; aptamer; enzyme-linked immunosorbent assay
黃曲霉毒素是一類主要由黃曲霉和寄生曲霉產(chǎn)生的次生代謝產(chǎn)物，具有致突變性、致畸性和致癌性，極易對大豆、花生和玉米等農(nóng)作物及其加工產(chǎn)品造成污染，是對人類健康威脅最大的霉菌毒素之一。（剩余8938字）