乙酰唑胺對小鼠動脈粥樣硬化斑塊及其鈣化作用

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[關(guān)鍵詞] 動脈粥樣硬化；血管鈣化；碳酸酐酶II；乙酰唑胺；小鼠，近交C57BL[中圖分類號] R361.1；R977.3 ［文獻(xiàn)標(biāo)志碼］A [文章編號] 2096-5532（2025）03-0376-05doi：10.11712/jms.2096-5532.2025.61.093 [開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）][網(wǎng)絡(luò)出版] https：//link.cnki.net/urlid/37.1517.R.20250724.1505.003；

2025-07-24 17：42：05

Effects of acetazolamide on atherosclerotic plaque formation and calcification in miceLIU Han ，AN Yi （Department of Cardiology，The Affiliated Hospital of Qingdao University，Qingdao 266oo3，China）

[Abstract]ObjectiveToinvestigate the efects of acetazolamideonatherosclerotic plaque formationand vascularcalcification in mice. MethodsFour-week-old Apoe-/-（n=3） and wild-type （ n=3 ） C57BL/6 mice were fed for 21 weeks，and subsequently，body weight and serum lipid levels were compared. Twelve l2-week-old Apoe-/- mice were divided into control group （ n=8 ，given normal saline） and experimental group （ ，given acetazolamide at 40mg/kg .After 2 weeks of treatment，aortic tissues wereharvestedtoevaluate plaquearea withHEstaining，characterizelipidcomposition withOilRedOstaining，measure calcium content using calcium assay kits，and determine the expression of carbonic anhydrase I （CA2）by Western blot. Results Apoe-/- mice exhibited significantly higher body weight and serum lipid levels than wild-type mice （F=24.15-61.24，t=2.891- 7.229，P<0.05） . At 3 months of age， plaque area，calcium content，and CA2 expression in aortic tissues were significantly increased in Apoe-/- mice （t=2.872-13.360，P<0.05） .Compared with controls，acetazolamide-treated Apoe-/- mice showed significantly reduced CA2 expresson，lipid-rich necrotic core area，and calcification severity （t=3.820-9.701，P<0.05） .ConclusionAcetazolamide attenuates atherosclerotic plaque formationand caleification by suppressing CA2 expressioninmice.

[Key words]atherosclerotic；vascular calcification；carbonic anhydrase II；acetazolamide；mice，inbred C57BL

冠心病是一種常見的心血管疾病，其病因是動脈粥樣硬化斑塊形成導(dǎo)致的血管狹窄與血管鈣化，全球每年因其而死亡病人高達(dá)1700萬[1]。（剩余8178字）