+</sup> )處理后N2a細(xì)胞線粒體數(shù)量的影響。方法采用Westernblotting法檢測(cè) MPP<sup>+</sup> 處理后 N2a 細(xì)胞中ACSL4的蛋白表達(dá)水平；向N2a細(xì)胞中轉(zhuǎn)染Flag-Vector或Flag-ACSL4質(zhì)粒，采用Westernblotting法檢測(cè) MPP<sup>+</sup> 處理后過(guò)表達(dá)ACSL4對(duì)N2a細(xì)胞中電壓依賴(lài)陰離子通道1（VDAC1)和線粒體通透孔蛋白20（TOMM20)表達(dá)水平的影響。結(jié)果與對(duì)照組相比，MPP<sup>+</sup> 處理組細(xì)胞中ACSL4蛋白表達(dá)水平顯著升高 (t=3.965,P<0.05) 。與對(duì)照組相比， MPP<sup>+</sup> 處理組細(xì)胞中VDAC1和TOMM20表達(dá)水平均升高，差異有顯著性 Δ<sup>′</sup>F=28.59,15.15,P<0.01) ；與 MPP<sup>+</sup> 處理組相比，過(guò)表達(dá)ACSL4并不會(huì)進(jìn)一步影響N2a細(xì)胞中VDAC1和TOMM20的表達(dá)水平（ ?P>0.05? 。結(jié)論過(guò)表達(dá)ACSL4對(duì)MPP<sup>+</sup> 處理后 N2a細(xì)胞線粒體數(shù)量無(wú)影響，提示ACSL4在帕金森病發(fā)病中所發(fā)揮的作用并不涉及對(duì)線粒體數(shù)量的影響。-龍?jiān)雌诳W(wǎng)" />

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ACSL4對(duì)MPP+處理后N2a細(xì)胞線粒體數(shù)量的影響

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[關(guān)鍵詞]輔酶A連接酶類(lèi);1-甲基-4-苯基吡啶;線粒體;電壓依賴(lài)性陰離子通道1;帕金森病[中圖分類(lèi)號(hào)］R338.2 [文獻(xiàn)標(biāo)志碼］A [文章編號(hào)] 2096-5532（2025）03-0327-04doi：10.11712/jms.2096-5532.2025.61.081 [開(kāi)放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）][網(wǎng)絡(luò)出版]https：//link.cnki.net/urlid/37.1517.R.20250716.1027.004; 2025-07-1615：08：55

Efectof acy-CoAsynthetaselong-chainfamilymember4onmitochondrialnumberinN2acelsafter1-methyl-4-phenylpyridinim treatmentLIU Siqi，LIU Mengru，JIANG Hong（State KeyDisciplines：Physiology （Incubation），Departmentof Physiolo gy，Qingdao University，Qingdao ，China）

[Abstract]ObjectiveToinvestigate theefectofacyl-CoA synthetaselong-chain family member4（ACSL4）onmitochondrial number in N2a cells after1-methyl-4-phenylpyridinium （ .MPP+ ）treatment. MethodsWestern blotting was used to measure the protein expression level of ACSL4 in N2a cells after MPP+ treatment. N2a cels were transfected with Flag-Vector or FlagACSL4 plasmids，and Westernbloting wasused toobservethe efect of ACSL4overexpressonontheexpressonlevelsofvoltage dependentanionchanel1（VDAC1）and mitochondrial import receptor subunit TOM20 homolog（TOMM2o）in N2acels after （20號(hào) MPP+ treatment. ResultsCompared with the control group，the MPP+ treatment group had a significant increase in the protein expression level of ACSL4 in N2a cells Ω′t=3.965，P<0.05） . Compared with the control group，the MPP+ treatment group had significant increases in the expresson levels of VDAC1 and TOMM20 （ F=28.59，15.15，P<0.01） ，while compared with the MPP+ treatment group，ACSL4 overexpression had no further impact on the expresson levels of VDAC1 and TOMM20 in N2a （204號(hào) cells （P>0.05 ）.ConclusionACSL4 overexpression has no impact on mitochondrial number in N2a cells after MPP+ treatment，sugestingthattheroleof ACSL4inthepathogenesisofParkinson’sdiseasedoes not involveitsimpactonmitochondrial number.

[Key words] coenzyme A ligases;1-methyl-4-phenylpyridinium; mitochondria;voltage-dependent anion channel 1； Parkinson disease

帕金森?。≒D）是一種常見(jiàn)的神經(jīng)退行性疾病，主要病理特征為中腦黑質(zhì)多巴胺能神經(jīng)元選擇性缺失，殘存的神經(jīng)元內(nèi)出現(xiàn)以 α- 突觸核蛋白（ ?a- Syn）異常聚集為主的路易小體[2]。（剩余8849字）

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