去亞甲基小檗堿對MPP+誘導(dǎo)的SH-SY5Y細(xì)胞線粒體功能損傷的保護作用

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[中圖分類號] R338.2;R742.5 [文獻標(biāo)志碼]A [文章編號]2096-5532(2025)03-0363-05doi:10.11712/jms.2096-5532.2025.61.098 [開放科學(xué)(資源服務(wù))標(biāo)識碼(OSID)][網(wǎng)絡(luò)出版] https://link.cnki.net/urlid/37.1517.R.20250730.1149.008; 2025-07-3014:09:52
Protectiveefectofdemethyleneberberineagainstmitochondrialinjuryinducedby1-methyl-4-phenylpyridiniuminSH-SY5Yclls GONG Yongxin,LI Caiteng,ZHU Tianli,MA Zegang(Departmentof Physiology,Scholof Basic Medicine,Qingdao University,Qingdao 266071,China)
[Abstract]ObjetiveToinvestigate theprotective efectof demethyleneberberine(DMB)against mitochondrial injuryin duced by 1-methyl-4-phenylpyridinium ( MPP+ )in SH-SY5Y cells.MethodsSH-SY5Y cells were divided into control group, (204號 MPP+ group,selegiline+MPP+ group,and DMB+MPP+ group. CCK8 assay was used to observe the effect of different concentrationsofDMBonthesurvivalrateofSH-SY5Ycels,andflowcytometrywasused to measure mitochondrialmembranepotential (2號 (Δψm) and reactive oxygen species (ROS).ResultsThere was a reduction in the survival rate of SH-SY5Y cels after treatment with 1 mmol/L MPP+ for 24 hours,and different concentrations of DMB could improve the survival rate of SH-SY5Y cells, among which the co-treatment group with 10-3μmol/L DMB and MPP+ had the highest survival rate ?F=74.5,q=18.12,P< 0.001). MPP+ treatment reduced Δψm in SH-SY5Y cells ?F=185.2,q=33.08,P<0.001) ,while both DMB and selegiline could reverse the effect of MPP+(q=12.97,15.32,P<0.001) 1 MPP+ treatment increased the content of ROS in cells ( ?F=79.2,q= : ?0.88,P<0.001 ),while DMB and selegiline eliminated the effect of MPP+(q=14.62,15.08,P<0.001) .Conclusion DMB can alleviate mitochondrial injury in SH-SY5Y cells induced by MPP+ :
[Keywords]berberine;Parkinson disease;mitochondria;selegiline
帕金森病(PD)作為一種神經(jīng)退行性疾病,常伴有靜止性震顫等癥狀,病理特征是中腦黑質(zhì)致密部多巴胺(DA)能神經(jīng)元的丟失等[1-2]。(剩余10384字)