MPTP和 α-Syn 預(yù)制原纖維誘導(dǎo)的慢性帕金森病模型小鼠黑質(zhì)多巴胺能神經(jīng)元的自發(fā)放電活動

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[中圖分類號] R742.5；R338.8 [文獻(xiàn)標(biāo)志碼］A [文章編號] 2096-5532（2025）03-0350-05doi：10.11712/jms.2096-5532.2025.61.082 [開放科學(xué)（資源服務(wù)）標(biāo)識碼（OSID）][網(wǎng)絡(luò)出版]https：//link.cnki.net/urlid/37.1517.R.20250716.0942.001; 2025-07-1615：10：45

oontaneousdischargesofnigraldopaminergicneurons inmousemodelsofchronicParkinson’sdiseaseinducedbyMPTPand α -Syn

PFFsLIU Wenhong，LIU Cui，CHEN Xinyi， XUE Yan， CHEN Lei（Department of Physiology and Pathophysiology， 5chool of Basic Medicine，Qingdao Medical College of Qingdao University，Qingdao 266o71，China）

[Abstract]ObjectiveToinvestigate thespontaneous activityof substantianigra dopaminergic neurons in twomouse models of chronicParkinson’sdiseaseinducedby1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine（MPTP）andalpha-syucleinprefored fibrils （ α Syn PFFs），respectively.MethodsTwo mouse models of chronic Parkinson’s disease were prepared bylong-term injection of MPTP into the peritoneal cavity and stereotaxic injection of α -SynPFFs into the right striatum of the brain，respectively. Werecorded thespontaneousfiringactivityofdopaminergicneurons inthesubstantianigra parscompactaof the two modelsusing electrophysiology techniques. ResultsThe MPTP group and α Syn PFFs group showed significantly smaller mean numbers of firing dopaminergic neurons per recording session in the substantia nigra pars compacta （t=3.015，4.779 ，both P<0.05 ），significantly higher firing rates of nigral dopaminergic neurons ，both P<0.05 ），and significantly higher proportions of nigral dopaminergic neurons exhibiting burst firing （t=-3.611，-2.928 ，both P<0.05 ），compared to the control group. ConclusionMouse models of chronic Parkinson’s disease induced by MPTP and α Syn PFFs differ significantly from control mice， mainly manifesting withdecreasednumbersofneuronsexhibiting spontaneousfiringandincreasedfiringratesand proportionsof neurons exhibiting burst firing.

[Key words]substantianigra；dopaminergic neurons;alpha-synuclein；-methyl-4-phenyl-1，2，3，6-tetrahydropyridine;elec trophysiology；Parkinson disease；mice

黑質(zhì)多巴胺能神經(jīng)元的進(jìn)行性缺失是帕金森?。≒D）的特征性病理改變之一[1-2]。（剩余10824字）