人參皂苷Rg1對(duì)TGF-β1誘導(dǎo)的星形膠質(zhì)細(xì)胞反應(yīng)性特性及細(xì)胞表型的影響

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[關(guān)鍵詞］人參皂苷 Rg1 ；星形膠質(zhì)細(xì)胞；轉(zhuǎn)化生長(zhǎng)因子β1；表型[中圖分類(lèi)號(hào)]R741;R284 [文獻(xiàn)標(biāo)志碼]A [文章編號(hào)] 2096-5532（2025）03-0345-05doi：10.11712/jms.2096-5532.2025.61.097 [開(kāi)放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）][網(wǎng)絡(luò)出版] https：//link.cnki.net/urlid/37.1517.R.20250730.1031.003; 2025-07-3014：11：53

Effect of ginsenosideRg1onTGF-β1-induced reactivityand phenotypeof astrocytesHULirong，CHEN Wenfang，ZHENG Yangyang （Department of Physiologyand Pathophysiology，Schoolof Basic Medicine，Qingdao University，Qingdao 266071，China）

[Abstract]ObjectiveTo investigate the efectof ginsenosideRglonthereactivecharacteristicsand phenotypic transformationofastrocytes（As）inducedbytransforminggrowthfactor-beta1（TGF-β1）.MethodsAs wereisolatedfromthecerebral cortex of neonatal Sprague-Dawleyratsandwereinducedintoreactiveastrocytes（RAs）byTGF-βl，andthenRAsweretreated with ginsenoside Rg1 for 12 or 24 hours. The experiment was divided into control group，TGF-βl group，and TGF- group. Quantitative real-time PCR was used to measure the mRNA expresion levels of reactivity markers （Gfap，S10O），inflammatory factors（ IL Iβ ，TNF-a，iNOS），Al-typeAs markers （Gbp2，H2–T23） ，and A2-type As markers （SIOOAIO，Ptx3） ，andWesternbloting wasused to measure thechangesintheprotein expresionlevelsofGFAPandiNOS.ResultsGinsenosideRglsignificantly reduced the mRNA expression levels of Gfap ， SIOO ，iNOS， TNF–α ，and IL–Iβ （ F=11.09-136.70，q=6.43-16.01 ， P<0.05 ）and the protein expression levels of GFAP and iNOS in RAs （F=8.67，7.66，q=6.80，5.36，P<0.05） . Compared with the control group，the TGF- β1+Rg1 group had significant reductions in the A1-type markers Gb?2 and H2-T23（ F=61.94 ， 18.46，q=13.95，5.58，P<0.05） and significant increases in the A2-type markers S1ooA1o and Ptx3 ？ ′F=25.46，31.11，q=7.70 ， （204號(hào) 10.65，P<0.01） .Compared with the TGF- ?β1 group，the TGF group had significant reductions in the Al-type markers Gbp2 and H2 -T23 ?F=61.94，18.46，q=13.29，8.45，P<0.01） ，with no significant changes in A2-type markers（SlooA10， （20 Ptx3） .Conclusion Ginsenoside Rgl can inhibit TGF- ?β1 -induced reactive characteristics of As and promotes their phenotypic transformation from A1 phenotype to A2 phenotype.

[Key words]ginsenoside Rg1 ；astrocytes; transforming growth factor β1 ；phenotyp！

星形膠質(zhì)細(xì)胞（As）作為中樞神經(jīng)系統(tǒng)（CNS）中主要的神經(jīng)膠質(zhì)細(xì)胞，具有維持血腦屏障、突觸形成與傳遞等作用[1-6]。（剩余10596字）