6-羥基多巴胺對(duì)BV2小膠質(zhì)細(xì)胞鐵蛋白表達(dá)的影響

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[關(guān)鍵詞] 羥多巴胺；鐵蛋白質(zhì)類；小神經(jīng)膠質(zhì)細(xì)胞；帕金森病[中圖分類號(hào)]R742.5；R341.3 ［文獻(xiàn)標(biāo)志碼］A [文章編號(hào)] 2096-5532（2025）03-0359-04doi：10.11712/jms.2096-5532.2025.61.078 [開(kāi)放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）][網(wǎng)絡(luò)出版]https：//link.cnki.net/urlid/37.1517.R.20250709.1559.005;

2025-07-10 11：55：34

Efect of 6-hydroxydopamineon the expressonofferitin inBV2 microglialcellsJING Cuiting，LIXiaoqian，QU Yan，XIE Junxia，WANG Jun（Departmentof Physiology，Scholof Basic Medicine，Qingdao University MedicalCollge，Qingdao 266071，China）

[Abstract]ObjetiveToinvestigate theeffctof6-hydroxydopamine（6-OHDA）ontheexpressionofferritin inBV2 microglialcell.MetodsAfterBV2microglialcelswere treated withdiffrentconentrationsof6-OHDA（1o，25，5Oμmol/L） for24hours，theamountoflactatedehydrogenase（DH）released was measured toreflectcellviability，andWesternblotwas usedtomeasurethechanges inthe protein expressionlevelsofferitin heavychain1（FTH1）andferrtinlightchain（FTL）in cells.ResultsCompared with thecontrol group without6-OHDA treatment，the BV2 microglial cels treated with 10μmol/L 6-OHDA for 24 hours had no significant change in the amount of LDH released，and the groups treated with 25 and 50μmol/L 6- OHDA and the maximum enzyme activity control group had a significant increase in the amount of LDH released ?F=92.750，q= （20號(hào) 6.799-24.780，P<0.001） . Compared with the control group，the BV2 microglial cells treated with 10μmol/L 6-OHDA for 24 hours had significant increases in the protein expression levels of FTHl and FTL （t=3.046，3.131，P<0.05） .ConclusionThis study shows that 6-OHDA can increase the expression of ferritin in BV2 microglial cells.

[Key words]oxidopamine；ferritins；microglia；Parkinson disease

帕金森?。≒D）是第二常見(jiàn)的中樞神經(jīng)系統(tǒng)退行性疾病[1]，黑質(zhì)鐵沉積是PD的重要病理表現(xiàn)[2]前期研究證實(shí)，在PD病人的中腦黑質(zhì)有大量激活的小膠質(zhì)細(xì)胞，這些細(xì)胞中存在大量鐵沉積[3]。（剩余10429字）