利用InDel分子標記鑒定新鄉(xiāng)小包23大白菜種子純度

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The seed purity identification of Chinese cabbage Xinxiangxiaobao 23 by InDel molecular marker

XUShijing'， WANG Xiaoling1， XIAO Yan'， YUANRanghua'， YANG Chen2， JI Keying2 （1.Xiniag Henan，China）

Abstract：Torapidlydetectthepurityofhybridseedsfromfrozendamaged plots intheoverwinteringseed production base of Xinxiangxiaobao23，ensure that the qualityof seeds entering the market meets standards，protect consumers rights，ad improvethe eficiencyofhybridseed screening，specific primers were selectedfrom12 pairsof InDelprimers for Chinesecabbage to distinguish Xinxiangxiaobao 23andits parents，and seed identification wascarriedout.The resultsshowedthatthree pairsof primersexhibitedco-dominance，amongwhichonepaircould effectivelydistinguish Xinxiangxiaobao23fromtheotherthreesimilarvarieties.This primerwas verifiedin108hybrid progenies，andthe purity identification result was 93.5% ，whichwas basically consistentwith the field identification result.In conclusion，the InDelmarkerBrID9ol05selectedinthis studycanbeusedforrapid identificationof seedpurityin Xinxiangxiaobao 23， promoting the healthy development of the seed industry.

Key words：Chinese cabbage;Xinxiangxiaobao 23;InDel molecular marker; Seed purity identification

大白菜（Brassica rapaL.ssp.pekinensis）屬于十字花科蕓臺屬植物，在我國廣泛種植，年種植面積多達180萬 hm2 ，北方地區(qū)，如山東、河北、河南等地成為大白菜的主產區(qū)。（剩余7247字）