g</sub> ,利用qRT-PCR測(cè)定煙盲蝽雌蟲羽化后不同時(shí)期 Vg 基因表達(dá)情況。結(jié)果表明僅取食小白菜或煙草的煙盲蝽若蟲均能發(fā)育至成蟲,存活率分別為 82.05% 和 57.41% ,取食小白菜能產(chǎn)生后代,而取食煙草無后代;添加蚜蟲后能顯著縮短煙盲蝽若蟲的發(fā)育歷期,并顯著提高煙盲蝽在煙草上的存活率和后代數(shù)量??寺〉玫降臒熋を?Vg 基因的編碼區(qū)(CDS)全長(zhǎng)5925bp,預(yù)測(cè)有4個(gè)保守結(jié)構(gòu)域。利用qRT-PCR技術(shù)檢測(cè)表明,取食小白菜的煙盲蝽羽化后第7天的Vg 基因表達(dá)量顯著高于第1天,而取食煙草的煙盲蝽 Vg 基因表達(dá)量沒有顯著變化;添加蚜蟲后, Vg 基因的表達(dá)水平均有顯著提高。小白菜可作為煙盲蝽擴(kuò)繁的寄主植物,且添加獵物蚜蟲有助于其生長(zhǎng)發(fā)育和繁殖。-龍?jiān)雌诳W(wǎng)" />

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不同寄主對(duì)煙盲蝽發(fā)育歷期和繁殖力的影響

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中圖分類號(hào):S435.72 文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):1007-5119(2025)04-0043-08

Abstract: Nesidiocoris emisisanimportant naturalenemyintobaccofield,whichcouldbeused tocontrol whiteflies,thripsand leaf miners etc.In this study,the effects offeding different plantson the growth,reproductionand survival of N. tenuis were studied with or without Myzus persicae. The vitellogenin ( (Vg) gene of N. tenuis was cloned, and the expression of Vg gene was determined by qRT-PCRat diferent periodsafter femaleemergence.Theresultsshowed thatthe nymphsfedonBrassicachinensisorNicotiana tabacum could develop to adults, and the survival rates were 82.05% and 57.41% , respectively. The nymphs fed on B . chinensis could produce offspring,while the nymphs fed on N. tabacum had no offspring. The addition of aphids could significantly shorten the development duration of nymphs,and significantly increase the survival rateand the numberof offspring of N. tenuis on N. tabacum. The coding region (CDS) of the Vg gene was 5925 bp in length,and it was predicted to have four conserved domains. The results of qRT-PCR showed that the expression of Vg in female adults fed on B chinensis was significantly higher on the 7th day than on the 1st day, while the expression of Vg in female adults fed on N. tabacum did not change significantly. After adding aphids, the expression level of Vg gene was significantly increased. B? chinensis can be used as the host plant for the propagation of N. tenuis,and the addition of prey aphids is helpful for its growth and reproduction.

Keywords:Nesidiocoris tenuis;Nicotiana tabacum; Brassica chinensis;developmentalduration; vitellogenin

盲蝽是一類兼具植食性和肉食性的雜食性昆蟲,按照取食特性,可分為植食肉食性(phytozoophagy)和肉食植食性(zoophytophagy)兩類[1-2]。(剩余16272字)

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