三角槭一步成苗及生根機制

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中圖分類號：S792.35 文獻標識碼：A 文章編號： 1000-4440（2025）07-1422-07

The one-step seedling formation and rooting mechanism of Acer buergerianum Miq.

YAN Kunyuan，LI Shushun， MA Qiuyue， DU Yiming， LI Qianzhong， ZHU Lu（Institute ofLeisure Agriculture，Jiangsu Academy of Agricultural Sciences，Nanjing 21oo14，China）

Abstract：TheAcer buergerianum，renowned for itsornamental foliageand extensive applications，possesses significantaestheticandeconomic value.To facilitatetherapidproductionofhigh-qualityseedlingsandachieveindustrial-scale propagation，this study employed stem segments with budsas explants to screenforoptimal colection times，sterilization methods，and medium formulations foraxillrybud induction androtinduction.A comprehensive isue culture rapid propagationsystemforAcerbuergerianum was established.Further investigationintotherotformation mechanisminvolvedcytologicalanalysisofadventitiousrootprimordiaandtheexpresionlevelsofkeygenesduringrootdevelopment.Thefindings indicated thata combinationof 75% ethanol disinfection for 2O seconds followed by 0.1% mercuric chloride disinfection for 15 minutes was the most efectivesterilization methodfor explants collected in June.Theoptimal medium foraxillry bud induction was MS+0.5mg/L indole-3-butyric acid（IBA），achieving an induction rate of 82.3% .For adventitious root induction，theoptimal medium was 1/2 MS + 0.6 mg/L IBA，resulting in a rooting rate of 88.7% with an average of 4.9rootsperplant.The initiation and growth of adventitiousroot primordiain Acerbuergerianum werecloselyassociatedwith auxinIBA.At aconcentrationof 0.6mg/L IBA，the formation of root primordia was significantly enhanced，and the relative expression levels of key genes Ab PIN1，AbPIN2，and AbPIN3 during root development increasedmarkedly.Thisstudyprovidesatechnicalreferenceforlarge-scalepropagationofnewvarietiesofAcerbuergerianum and establishes afoundation for exploring its root formation mechanism.

Key words： Acer buergerianum Miq.；tissue culture；rapid propagation；rooting

三角槭（AcerbuergerianumMiq.）為無患子科槭屬落葉喬木，高度可達 10m ，樹冠卵形，原產(chǎn)于中國東部[1-2]。（剩余10170字）