重樓全長(zhǎng)轉(zhuǎn)錄組測(cè)序及生物信息學(xué)分析

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Full-length transcriptome sequencing and bioinformatics analysis of Paris

XU Ping'，ZHANG Xuan’，YU Mengwen1，XU Honggao1，TU Zhenhua’，ZHANG Xiaoye'

ZHANG Jie'， OU Zhenggang2， CHEN Jia3， ZHENG Guowei1

Abstract：The purpose of this study is to lay a foundation for the systematic exploration of saponin synthesis genesin Paris byobtaining the full-length transcriptome data of Paris. The third-generation sequencing technologyon PacBio Sequel platform was used.In this study，the rhizome mixed samples of Paris polyphylla Smithvar.yunnanensis（Franch.）Hand.-Mazz.，Paris fargesiiFranch.andParisforrestii（Takht.）H.Liwith significantdifferences in saponin content were deeply sequenced，and then the obtained data were comprehensivelyanalyzedbybioinformatics.A total of42771 transcriptswereobtained，of which40945（ 95.73% ）were successfully matched to seven functional databases，namely KEGG，NR，Swiss-Prot，TrEMBL，KOG，GOand Pfam.Through enrichmentanalysisofKEGG pathway，48genesrelatedtocholesterol synthesis were successullyidentified，andtwokindsofkey enzyme genes involved insaponin modification were specifically screened：12O cytochrome P45O enzyme genes and 53 UDP-glucosyltransferasegenes.Additionaly，2327transcriptionfactorsweredetected，belonging to71transcriptionfactor families.Atotalof27451microsatelitesequences（SSR）werefoundbyMISAanalysis，therewerethemostcoincident types with overlapping SSR，accounting for 32.88% of all repeat types.With the help of high-throughput full-length transcriptome sequencing technology，thetranscriptomeof rhizome tisueof Pariswassystematicallyanalyzed，and thecandidategens andregulatoryelements closelyrelated tosaponinsynthesis weresuccessfully excavated.Thesefindings laythe groundwork for further study onthe molecular mechanism of saponin biosynthesis in Paris.

Key words： Paris；full-length transcriptome；function annotation；saponin

重樓屬（ParisL.）植物為藜蘆科（Melanthiace-ae）多年生草本植物。（剩余14706字）