三七總皂苷對(duì)大鼠心肌缺血再灌注損傷的影響及抗焦亡機(jī)制研究
打開文本圖片集
doi：10.12102/j.issn.1672-1349.2025.14.007
EfectsofPanaxNotoginsengSaponinsonMyocardiallschemia-reperfusionInjuryinRatsandtheMechanismofAnti-pyroptosis ZHAOWeikun1，UTongtong'，UXiangwe，QNYang1，LUFengxia1，Yuting2，HUANGYul，OQ，UHuaxin，Ue
1.TheFirst AfaesiafuindaUveitunag，ina;uiicavsit99 Guangxi， China
Corresponding AuthorQINYang，E-mail：1258778506@qq.com;LYU Xiangwei，E-mail：lvxiangwei910 @ 163.com
AbstractObjective：ToobservetheefectofPanaxnotoginsengsaponins（PNS）onmyocardialischemia-repefusion（I）injuryand exploreitsmechanismrelatedtotheregulationofcardiomyocytepyroptosis.Methods：EighteenadultmaleSDratsweredividedintosham group，IR groupandPSgroupbyrandomnumbertablemethod，with6ratsineach group.Themodelwaspreparedbyligatigtheleft anteriordescendingcoronaryarteryAD）ofratswithischemiafor30minutesandreperfusionfor2hours.Theatsinthesamgroupand the IR groupwere intraperitoneally injectedwith normal saline（ 10mL/kg） ，while those in the PNS groupwere intraperitoneally injected with （20 PNS（50mg/kg） 30 minutes before modeling.The degree of myocardial injury was observed by hematoxylin-eosin（HE） staining.The expresionofcsteineaspartatespecicprotease-1Caspase1）asdetectedyimmunoistochemistryeexpresiosofCaspse1 andgasdermin D（GSDMD）proteincontentsinmyocardialtisseweredetectedbyWesternBlotResuts：Comparedwiththeshamgroup， thedegree ofmyocardialinjuryintheIRgroupwasaggravated，andtheproteinexpressionsofaspase-1andGDinceased P< 0.05）.ComparedwiththeIRgroup，thedegreeofmyocardialinjuryinthePNSgroupwasaleviatedandtheproteinexpressionsof Caspase-1 and GSDMD decreased（ ?∠1.5′ .Conclusion：Panax notoginseng saponins could alleviate myocardial iR injury in rats，and its mechanism might relate to the inhibition of cardiomyocyte pyroptosis.
Keywordsmyocardialiscemiareperfusioninjuypanaxotogisengsapons;pyptosis;csteineaspasticaidspeificote1 （Caspase-1） ; gasdermin D（GSDMD）; rats; experimental study
急性心肌梗死（acutemyocardial infarction，AMI）發(fā)生時(shí)，早期治療可恢復(fù)缺血心肌的血液供應(yīng)，降低死亡風(fēng)險(xiǎn)；持續(xù)的缺血狀態(tài)導(dǎo)致心肌細(xì)胞出現(xiàn)不可逆性損害。（剩余9283字）