高壓靜電場聯(lián)合蓮房原花青素對常見腐敗菌致冷藏魚片品質(zhì)劣變的抑制作用

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Abstract：Toinvestigatethedegradationoffishproteinbydominantspoilagebacteriaandtheinhibitoryeffectofcombined treatment with high-voltage electric field （HVEF）and lotus seedpod procyanidins （LSPC）onquality deteriorationof fish meatinfish filets，sterilecatfish flets treated withHVEFandLSPCwere inoculated individuallywith three majorspoilage bacteria，Shewanella putrefaciens，Shewanella havnia and Serratiasp.，and stored at 4°C . Total viable count （TVC）， total volatile basic nitrogen （TVB-N） content，and trichloroacetic acid （TCA）soluble peptidecontent were measured during storage，along with free aminoacidcontents，biogenicamines （BAs）contentsand transcriptional levelsof putrescine metabolism genes after storage for 10d We explored the effects of the three spoilage bacteria on the quality of refrigerated catfishflletsandtheinibitoryefectofHVEFcombined withLSPContheirspoilageability.Theresultsshowedthatafter storagefor10d，theTVCoffletsinoculatedwithS.putrefaciens，S.hani，andSerratiasp.decreasedby3.08，.6and

2.58 （log（CFU/g）） compared with the untreated control group，respectively.The TCA soluble peptide contents decreased by 0.33，0.60， 0.59μmol/g ，the TVB-N content declined by 33.81% ， 25.19% 22.98% ，and the total BA contents （including putrescine and cadaverine） significantly reduced （P<0.05 ）.Additionally，the transcription levels of the putrescine formation-related genes speA and speBwere significantly down-regulated （P<0.05 ）.HVEFcombinedwithLSPC effectively inhibited the spoilage activity ofspoilage bacteria and delayed protein degradation in fish.

Keywords：high-voltageelectostaticfield;lotusseedpod; procyanidins;spoilage bacteria; channelcatfsh;biogenicamines DOI：10.7506/rlyj1001-8123-20250216-044

中圖分類號：TS254.4引文格式：

文獻標志碼：A 文章編號：1001-8123（2025）08-0043-07 李亞俐，田尹瓊，石柳，等.高壓靜電場聯(lián)合蓮房原花青素對常見腐敗菌致冷藏魚片品質(zhì)劣變的抑制作用[J].肉類研 究，2025，39（8）： 43-49.DO1：10.7506/rlyj1001-8123-20250216-044. http：//www.rlyj.net.cn LI Yali，TIANYinqiong，SHLiu，etal.Inhibitoryefectofhigh-voltageelectrostaticfieldcombinedwithlotusseedpod procyanidinsonqualitydeteriorationcausedbycommonspoilage bacteriainrefrigeratedchannelcatfish[J].MeatResearch， 2025，39（8）：43-49.（in Chinese withEnglishabstract）DOI：10.7506/rlyj100-8123-20250216-044.htp：//www.rlyj.net.cn

斑點叉尾（Ictaluruspunctatus）又稱魚，原產(chǎn)于北美洲，于1984年引入中國[1]，以其優(yōu)異的感觀品質(zhì)和營養(yǎng)特性（富含優(yōu)質(zhì)蛋白、不飽和脂肪酸及礦物質(zhì)等）而備受消費者歡迎。（剩余15701字）