不同pH值下蛋白氧化和NaCI濃度對(duì)牦牛肉肌原纖維蛋白結(jié)構(gòu)的影響

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Abstract： In thisstudy，theeffectsof proteinoxidationandNaClconcentrationonthestructureofyak myofibril protein（MP） wereinvestigated.Tothisend，MPwasoxidizedfor1hinaFentonoxidationsystemandtreatedfor1hwithdierentNaCl concentrations under three pH values （5.0，6.O and 8.O），respectively.The total sulfhydryl content，surface hydrophobicity， solubility，sodiumdodecylsulfate-polyacrylamidegelelectrophoresis （SD-PAGE）pate，andmicrostructureof treated MP were determined.Theresultsshowed thatatafixed pH，the total sulfhydrylcontent and solubility decreased with increasing H2O2 concentration，and the surface hydrophobicity increased.In addition，the degree of expansion declined horizontally.Atafixed H2O2 concentration，the total sulfhydryl content and solubility increased with increasing pH ，and the surface hydrophobicitydecreased，accompaniedbylateral expansion，narrowingoftheA-bandanddestructionof the Z-line. At a fixed pH ，the solubility increased with increasing NaCl concentration，and the surface hydrophobicity decreased， accompaniedbylateralexpansionanddestructionofthetransverse bridge.AtafixedNaClconcentration，thesolubility increased with increasing pH ，thesurface hydrophobicity decreased，and lateral expansion occurred.At pH8.0 and 1mol/L NaClconcentration，boththeM-lineandZ-line wereobviouslyestroyed，thestructureofMbecameblurred，theyotome

was broken，thetransverse bridgewas destroyed，andcoarsefilaments were separated from fineones.Inconclusion，both protein oxidation and ΔNaCl had adverse effects onyak MP under different pH conditions，and protein oxidation had the lowest negative impact on MP structure at pH6.0 and a ΔNaCl concentration of 0.4mol/L ：

Keywords： myofibrillar protein; protein oxidation; pH value; NaCl concentration; protein structure

DOI：10.7506/rlyj1001-8123-20241028-284

中圖分類(lèi)號(hào)：TS251.1 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1001-8123（2025）06-0001-08 引文格式：

孫棟，胡婷，馮耀文，等.不同pH值下蛋白氧化和NaCI濃度對(duì)耗牛肉肌原纖維蛋白結(jié)構(gòu)的影響[J].肉類(lèi)研究，2025， 39（6）：1-8.DOI：10.7506/rlyj1001-8123-20241028-284. http：//www.rlyj.net.cn

SUN Dong，HU Ting，F(xiàn)ENG Yaowen，etal.Efectsof protein oxidationand NaClconcentrationonthe structureof yak myofibrillar proteinunderdifferentpHvalues[J].MeatResearch，2025，39（6）：1-8.（inChinesewith Englishabstract） DOI：10.7506/rlyj1001-8123-20241028-284.htp：//www.rlyj.net.cn

牦牛被譽(yù)為“高原之舟”，主要生長(zhǎng)在空氣稀薄、氣候寒冷的高海拔地區(qū)。（剩余13685字）