滇牡丹花瓣色斑形成的分子機(jī)制研究

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中圖分類號：Q943 文獻(xiàn)標(biāo)識碼：A 文章編號：1000-3142（2025）04-0628-13

Molecular mechanism of the colour spot formation in Paeonia delavayi petals

LI Haiqing'，DU Chun2，WANG Juan2*， ZHANG Huaibi3（1.ColegeofditUniversityKungO，a；3.ZedIsiutefornt&odsechLiedalestoto，Zd）

Abstract：To investigatethe molecular mechanisms of colour spotformation inPaeonia delavayi petals，this study used yellow petals of P .delavayi with and without colour spot as experimental material.Utilizing the Ilumina platform for transcriptome sequencing and the UPLC system for metabolome analysis，the keydiferentially expressed genes （DEGs）

and transcription factors affecting colour spot formation in P .delavayi were screened. The resultswere as follows：（1） The transcriptome sequencing yielded 63 981 Unigenes with an average length of 8 0 5 b p ，and 6 8 . 2 4 % of these Unigenes wereannotated.Atotalof19496DEGs wereidentified，of which41DEGs were involved inflavonoidbiosynthesisand DFR，CHS，and C H I structural genes showed significant differential expression. Among the obtained 37 MYB transcription factors，oneR2R3-MYB transcription factor，PdMYB3O，was found to playa significant role in promoting colour spot formation.（2）Targeted analysis using the UPLC-MS/MS platform detected 44 anthocyanin compounds.（3） The expresion trends of DEGs identified by RNA-seq were consistent with theqRT-PCR results.Insummary，the colour spot formation of P .delavayi is mainly influenced by anthocyanins.The transcription factor PdMYB3O ispositively correlated with the structural genes CHS， C H I ，and DFR during the yellow flower with red spot bud stage（B-S1） due to their similar highexpressonlevels.Itispredicted that PdMYB3Omayactasa positiveregulator in theflavonoid biosynthesispathway，enhancing theexpression levelsof structural genesinvolved inflavonoidbiosynthesis，thereby promoting theacumulationofanthocyanins intheplant.Thisstudyprovidesascientificbasis fordeveloping efficient breeding techniques for P .delavayi.

Key words：Paeonia delavayi，colour spot formation，transcriptome，metabolome，diferentially expressed genes （DEGs）

芍藥屬植物作為世界范圍內(nèi)享有盛譽(yù)的觀賞植物，其來源于中國，擁有源遠(yuǎn)流長的栽培歷史和育種傳統(tǒng)（Zhouetal.，2014）。（剩余21930字）