基于A u@Ag 納米粒子的氨基酸表面增強(qiáng)拉曼散射檢測(cè)
中圖分類號(hào):0657.37文獻(xiàn)標(biāo)志碼:A
Surface-enhanced Raman scattering detection of amino acids based on Au @ Ag nanoparticles
CHANGMin,LIU Huifang,SHI Yueyan,ZHANG Jiachang,LI Shibo,HE Chen (School of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093,China)
Abstract: Surface-enhanced Raman scattering (SERS) is widely employed for amino acids quantitative analysis due to its high-sensitivity molecular feature extraction and multiplex detection advantage. Stable and highly sensitive SERS substrates are crucial for achieving precise detection. Gold core silver shell nanoparticles (Au@Ag NPs) combine the chemical stability of gold nanoparticles with the high SERS enhancement effect of silver nanoparticles.They enable the screening and detection of seven amino acids, including phenylalanine and alanine. Except for isoleucine and glycine, which exhibited relatively lower sensitivity,the detection limits for the remaining amino acids reached 1×10-4mol/L . A linear correlation existed between concentration and SERS signal intensity, with correlation coefficients exceeding 0.94. This study provides a reference method for simultaneous quantitative detection of multiple amino acids on a single substrate.
Keywords: amino acid; surface-enhanced Raman scatering; Au@Ag NPs; quantitative detection
引言
氨基酸是構(gòu)成蛋白質(zhì)的基本單元,在人體生命活動(dòng)中發(fā)揮重要作用,因此對(duì)必需氨基酸需保證日常攝人。(剩余9078字)
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