基于PCR-CRISPR/Cas12a技術(shù)的腸炎沙門菌快速檢測(cè)方法

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中圖分類號(hào)：S852 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1001-411X（2025）03-0311-08

A fast method for detecting Salmonella enteritidis based on PCR-CRISPR/Cas12a

YANG Tianmut， WANG Yiheng?， XIONG Wenguang， GUO Jianying （Guangdong ProvincialKeyLaboratoryofVeterinaryPharmaceuticsDevelopmentandSafetyEvaluation/NationalLaboratoryof Safety Evaluation （Environmental Assessment） of Veterinary Drugs/National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria/College of Veterinary Medicine，South China Agricultural University， Guangzhou 510642， China）

Abstract： 【Objective】To realize early detection， preventionand control of Salmonela enteritidis，a detection method was established by combining CRISPR（Clustered regularly interspaced short palindromic repeat） technology with polymerase chain reaction （PCR）.【Method】 PCR primers were screened based on the conserved sdfI gene sequence. The sensitivity test was conducted using S. enteritidis genomes at diferent concentrations，whilethe specificitytests wereconducted using thegenomes ofEscherichiacoli，Staphylococcus aureus，Pasteurellamultocida，amonellacholeraesuisandEnterococcusfaecalis.Subsequently，thedeveloped method was applied for the detection of S ， enteritidis in small intestinal samples. 【Result】 This method was capable of identifying S. enteritidis in small intestinal samples， with a detection limit of ，showing excellent specificity and no cross-reactivity with E coli，S.aureus，P.multocida，S.choleraesuisand E faecalis 【Conclusion】 This study develops a compact diagnostic method for the early detection of S . enteritidis， showing excellnt sensitivity and specificity. This innovation presents a fresh perspective for the swift identificationofS.enteritidis.

Key words： Salmonella enteritidis; sdfI gene; PCR; CRISPR/Cas12a system

禽肉和禽蛋是人類優(yōu)質(zhì)蛋白質(zhì)的重要來源，禽生長(zhǎng)迅速、出欄較快，在中國(guó)居民膳食的動(dòng)物性食物中占較大比例。（剩余11923字）